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Column temperature was kept at 40 °C, flow rate 0.4 mL per min, and the solvent gradient was as follows: 0–9 min from 95% B to 70% B, 9-13 min from 70% B to 30% B, and 13-14 min held at 30% B followed by a 20 min re-equilibration gradient to prep the column before the next sample injection

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pathology 184 (1), 141–147